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PubMed · 12856095

Screen-printed multienzyme arrays for use in amperometric batch and flow systems.

S Sapelnikova·E Dock·R Solná·P Skládal·T Ruzgas·J Emnéus
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Abstract

Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.

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AcetylcholinesteraseBiosensing TechniquesCatecholsCholinesterasesElectrochemistryElectrodesEnzymes, ImmobilizedFlow Injection AnalysisHorseradish PeroxidaseHydrogen PeroxideMonophenol MonooxygenaseMultienzyme ComplexesPhenolsReproducibility of ResultsWater Pollutants, ChemicalJournal ArticleResearch Support, Non-U.S. Gov't

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S Sapelnikova, E Dock, R Solná, P Skládal, T Ruzgas, J Emnéus. 2003-07-11. Screen-printed multienzyme arrays for use in amperometric batch and flow systems.. https://doi.org/10.1007/s00216-003-2000-4

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