Oxygen supply modulates MCP-1 release in monocytes from young and aged rats: decrease of MCP-1 transcription and translation is age-related.

M Reale; C Di Giulio; M Cacchio; R C Barbacane; A Grilli; M Felaco; G Bianchi; M Di Gioacchino; P Conti

doi:10.1023/a:1024154704469

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PubMed · 12870648

Oxygen supply modulates MCP-1 release in monocytes from young and aged rats: decrease of MCP-1 transcription and translation is age-related.

M Reale·C Di Giulio·M Cacchio·R C Barbacane·A Grilli·M Felaco·G Bianchi·M Di Gioacchino·P Conti

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Abstract

Hyper or hypoxia may affect the immune system's chemokine production. Monocyte chemotactic protein-1 (MCP-1), an important chemotactic cytokine can be activated by active oxygen species. Groups of rats were exposed to hypoxic and hyperoxic environmental conditions for 60 h and MCP-1 was determined in their peripheral blood mononuclear cells by Elisa and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). In this study we evaluated if the ability of monocytes to produce MCP-1 under basal conditions or after stimulation with lipopolysaccharide (LPS) or phytohaemagglutinin (PHA) was differently affected by exposure to hyper or hypoxic conditions in young and aged rats. MCP-1 expression and production in monocyte/macrophages from rats at normoxic conditions was reduced in aged subjects. However, spontaneous, LPS or PHA-induced MCP-1 production was up-regulated by exposure to hyperoxic conditions in both young (62 +/- 8, 99 +/- 7, 102 +/- 8 pg/ml, respectively) and aged rats (79 +/- 4, 112 +/- 9, 117 +/- 10 pg/ ml, respectively). We conclude that hyperoxia is an important regulator of MCP-1 release and support the hypothesis that increased % of O2 may serve to initiate MCP-1 production which then serves to recruit and regulate the distribution of mononuclear cells to the sites of inflammation.

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Aging Animals Chemokine CCL2 Hypoxia Leukocytes, Mononuclear Male Monocytes Oxygen Protein Biosynthesis Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Time Factors Transcription, Genetic Journal Article

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M Reale, C Di Giulio, M Cacchio, R C Barbacane, A Grilli, M Felaco, G Bianchi, M Di Gioacchino, P Conti. 2003. Oxygen supply modulates MCP-1 release in monocytes from young and aged rats: decrease of MCP-1 transcription and translation is age-related.. https://doi.org/10.1023/a%3A1024154704469

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