Fluorescence-aided caries excavation (FACE) compared to conventional method.

Abstract

A recent study showed that orange-red fluorescence in carious dentin could be used to detect residual caries (Lennon & others, 2002). This study compared the ability of a new fluorescence-aided caries excavation technique (FACE) with the conventional method. Forty extracted teeth with occlusal dentin caries were selected. The teeth were bisected longitudinally through the center of the lesion. Lesion depth and width were measured and the teeth were divided into two groups of 20, each with the same average lesion size. The tooth halves were reassembled and fixed by embedding the roots in acrylic resin. Access cavities were prepared using a high-speed handpiece and diamond fissure bur. In the FACE group, violet light (370-420 nm) was fed into the fiber optics of a slow-speed hand-piece, so that it illuminated the operating field. The cavity was observed through a 530-nm high-pass filter and orange-red fluorescing areas were removed. In the conventional group, a sharp probe was used to detect soft dentin, which was removed. One-half of each tooth was stained for bacteria using Ethidium Bromide and examined using Confocal Laser Scanning Microscopy (CLSM). Bacteria were present in significantly (p=0.037) fewer FACE samples (3) compared to conventional samples (9). It can be concluded that the new method is more effective than conventional caries excavation.