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Total protein measurement using a fiber-optic evanescent wave-based biosensor.

P V Preejith·C S Lim·A Kishen·M S John·A Asundi
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Abstract

A novel method and instrumental system to determine the total protein concentration in a liquid sample is described. It uses a fiber optic total protein sensor (FOPS) based on the principles of fiber optic evanescent wave spectroscopy. The FOPS applies a dye-immobilized porous glass coating on a multi-mode optical fiber. The evanescent waves at the fiber optic core-cladding interface are used to monitor the protein-induced changes in the sensor element. The FOPS offers a single-step method for quantifying protein concentrations without destroying the sample. The response time and reusability of the FOPS are evaluated. This unique sensing method presents a sensitive and accurate platform for the quantification of protein.

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Biosensing TechniquesCytochrome c GroupEquipment Failure AnalysisFiber Optic TechnologyHemoglobinsOptical FibersOvalbuminProteinsReproducibility of ResultsRosaniline DyesSensitivity and SpecificitySerum Albumin, BovineSolutionsSpectrum AnalysisEvaluation StudyJournal ArticleValidation Study

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P V Preejith, C S Lim, A Kishen, M S John, A Asundi. 2003. Total protein measurement using a fiber-optic evanescent wave-based biosensor.. https://doi.org/10.1023/a%3A1021955032291

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