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Real-time PCR method to detect Enterococcus faecalis in water.

Jorge W Santo Domingo·Shawn C Siefring·Richard A Haugland
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Abstract

A 16S rDNA real-time PCR method was developed to detect Enterococcus faecalis in water samples. The dynamic range for cell detection spanned five logs and the detection limit was determined to be 6 cfu/reaction. The assay was capable of detecting E. faecalis cells added to biofilms from a simulator of a water distribution system and in freshwater samples. Nucleic acid extraction was not required, permitting the detection of E. faecalis cells in less than 3 h.

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DNA, BacterialEnterococcus faecalisEnvironmental MonitoringPolymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityWaterWater MicrobiologyWater PollutionEvaluation StudyJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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Jorge W Santo Domingo, Shawn C Siefring, Richard A Haugland. 2003. Real-time PCR method to detect Enterococcus faecalis in water.. https://doi.org/10.1023/a%3A1022303118122

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