Recombinant mannan-binding lectin (MBL) for therapy.

J C Jensenius; P H Jensen; K McGuire; J L Larsen; S Thiel

doi:10.1042/bst0310763

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Recombinant mannan-binding lectin (MBL) for therapy.

J C Jensenius·P H Jensen·K McGuire·J L Larsen·S Thiel

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Abstract

Mannan-binding lectin (MBL) is a plasma protein involved in the innate immune response. It binds to a number of micro-organisms and promotes killing of these through complement activation either directly or through opsonization. Clinical evidence indicates that in a variety of situations genetically determined low MBL levels are associated with increased susceptibility to infections. Infusions of plasma-derived MBL into MBL-deficient individuals was found to be safe in preliminary trials, but we considered that sufficient production and product safety could only be achieved through synthesis of recombinant MBL. A transfected human cell line produces MBL showing the same biological activity as plasma-derived MBL, and an essentially identical profile on MS. The production has been scaled up and clinical trials will start this year.

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Animals Cell Line Cold Temperature Complement Activation Complement C4 Drug Stability Humans Mannose-Binding Lectin Recombinant Proteins Reference Values Serine Endopeptidases Time Factors Comparative Study Journal Article Review

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J C Jensenius, P H Jensen, K McGuire, J L Larsen, S Thiel. 2003. Recombinant mannan-binding lectin (MBL) for therapy.. https://doi.org/10.1042/bst0310763

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