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PubMed · 12892882

New integration vector using a cellulase gene as a screening marker for Lactobacillus.

Soo Jin Jang·Min Seok Ham·Jung Min Lee·Sung Kyun Chung·Hyong Joo Lee·Jeong Hwan Kim·Hae Choon Chang·Jong-Hoon Lee·Dae Kyun Chung
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Abstract

The new integration vector for Lactobacillus, pJC4, was developed using the extracellular endoglucanase A gene (celA) of Clostridium thermocellum as a screening marker. pJC4 was transformed into four Lactobacillus species, Lb. johnsonii, Lb. gasseri, Lb. bulgaricus, and Lb. plantarum. In each species, the pJC4 integrants were easily and accurately detected by the appearance of a clear halo on a cellulase screening plate without any false transformants. Polymerase chain reaction and Southern hybridization indicated that all transformants with clear halos contained pJC4 in their chromosomal DNAs. The celA gene could be a useful screening marker for other lactic acid bacteria.

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CellulaseCloning, MolecularGenetic TestingLactobacillusMicrobiological TechniquesTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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Soo Jin Jang, Min Seok Ham, Jung Min Lee, Sung Kyun Chung, Hyong Joo Lee, Jeong Hwan Kim, Hae Choon Chang, Jong-Hoon Lee, Dae Kyun Chung. 2003-07-29. New integration vector using a cellulase gene as a screening marker for Lactobacillus.. https://doi.org/10.1016/s0378-1097(03)00422-1

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