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Substrate recognition by the human MTH1 protein.

Hiroyuki Kamiya·Laurence Dugué·Hiroyuki Yakushiji·Sylvie Pochet·Yusaku Nakabeppu·Hideyoshi Harashima
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Abstract

A nucleotide pool sanitizing enzyme, the human MTH1 protein, hydrolyzes 2-hydroxy-dATP, 8-hydroxy-dATP, and 8-hydroxyd-GTP. To examine the substrate recognition mechanism of the MTH1 protein, ten nucleotide analogs (8-bromo-dATP, 8-bromod-GTP, deoxyisoinosine triphosphate, 8-hydroxy-dITP, 2-aminopurine-deoxyriboside triphosphate, 2-amino-dATP, deoxyxanthosine triphosphate, deoxyoxanosine triphosphate, dITP, and dUTP) were incubated with the protein. Of these, the former five nucleotides were hydrolyzed with various efficiencies. This results suggests the importance of the anti/syn-conformation and the functional groups on the 2 and 6-positions of the purine ring.

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DNA Repair EnzymesHumansNucleotidesPhosphoric Monoester HydrolasesSubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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Hiroyuki Kamiya, Laurence Dugué, Hiroyuki Yakushiji, Sylvie Pochet, Yusaku Nakabeppu, Hideyoshi Harashima. 2002. Substrate recognition by the human MTH1 protein.. https://doi.org/10.1093/nass%2F2.1.85

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