NMR study of a novel RNA quadruplex structure.

H Liu; M Kanagawa; A Matsugami; Y Tanaka; M Katahira; S Uesugi

doi:10.1093/nass/44.1.65

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NMR study of a novel RNA quadruplex structure.

H Liu·M Kanagawa·A Matsugami·Y Tanaka·M Katahira·S Uesugi

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Abstract

The structure of an RNA oligomer, r (GGAGGUUUUGGAGG) (R14-2) whose G-G steps are separated by adenine and uracil residues has been investigated by NMR. In the presence of 20 mM K+, a novel dimeric multiplex architecture is adopted by two strands of R14-2. In each strand a UUUU loop and two A residues connect four parallel G-G steps that pair-align into two tetrads. One of the tetrads is further pair-aligned by two A residues through the sheared mismatch and a novel hexad is subsequently formed. Two hexads coming from two different strands stack to make a dimeric multiplex. All of the guanosine and adenosine residues take an anti conformation.

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Base Sequence Models, Molecular Nuclear Magnetic Resonance, Biomolecular Nucleic Acid Conformation Oligoribonucleotides RNA Journal Article Research Support, Non-U.S. Gov't

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H Liu, M Kanagawa, A Matsugami, Y Tanaka, M Katahira, S Uesugi. 2000. NMR study of a novel RNA quadruplex structure.. https://doi.org/10.1093/nass%2F44.1.65

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