Solid-phase synthesis of deoxyribonucleotides having a cap structure analog.

M Ushioda; M Kadokura; Y Murami; T Moriguti; T Wada; K Seio; M Sekine

doi:10.1093/nass/44.1.123

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Solid-phase synthesis of deoxyribonucleotides having a cap structure analog.

M Ushioda·M Kadokura·Y Murami·T Moriguti·T Wada·K Seio·M Sekine

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Abstract

In this study, the solid-phase synthesis of oligodeoxyribonucleotides having a guanosine pyrophosphate cap structure (Gpp-) was achieved by using a new guanosine monophosphate unit having the DMTr group capable of estimation of coupling efficiency of the pyrophosphate bond formation. Since 7-methylguanosine base was unstable under basic conditions, Gpp-capped DNA oligomers were synthesized by using a new linker having a silanediyl bond, which allowed to release the DNA chain from the solid support by treatment with fluoride anion under neutral conditions.

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Methods Molecular Structure Oligodeoxyribonucleotides RNA Cap Analogs Journal Article

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M Ushioda, M Kadokura, Y Murami, T Moriguti, T Wada, K Seio, M Sekine. 2000. Solid-phase synthesis of deoxyribonucleotides having a cap structure analog.. https://doi.org/10.1093/nass%2F44.1.123

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