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PubMed · 12903784

Study on modified cold storage method of rat livers with self-made hyd solution.

B Sun·H Jiang·H Qiao·D Piao·J Sun·S Zhu·X Wang
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Abstract

OBJECTIVE: To investigate the cold preservation effect of rat livers by modified storage method with self-made HYD solution. METHODS: The modified method was that the vascular bed of rat livers was expanded with an additional 20 to 40ml self-made HYD solution/100g liver. After removing the liver, the extra HYD solution expressed as % liver weight was entrapped via portal infusion by tying off the supra-and infra hepatic inferior vena cava. According to the amount of extra HYD solution, 40 rats were randomly divided into four groups including: control group with conventional storage method, 20% group, 30% group and 40% group. The preservation effect of modified storage method with that of conventional storage method by using isolated perfused rat liver model was compared. RESULTS: Bile production and all the indices of hepatic microcirculation including portal perfusion pressure, endothelin-1 in the effluent, trypan blue distribution time and histology in modified method groups were significantly superior to those in control group (P < 0.05). The liver enzymes in 30% group were markedly lower than those in control group (P < 0.05). The preservation effect of rat liver in 30% group was the best among the modified method groups. CONCLUSION: The modified cold stage method is effective and may have potential for clinical application for liver preservation.

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Alanine TransaminaseAnimalsAspartate AminotransferasesBileCold TemperatureEndothelin-1L-Lactate DehydrogenaseLiverMaleOrgan PreservationOrgan Preservation SolutionsPerfusionPortal VeinPressureRatsRats, WistarJournal ArticleResearch Support, Non-U.S. Gov't

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B Sun, H Jiang, H Qiao, D Piao, J Sun, S Zhu, X Wang. 2000. Study on modified cold storage method of rat livers with self-made hyd solution.. https://pubmed.ncbi.nlm.nih.gov/12903784/

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