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Optical sectioning by two-pinhole confocal fluorescence microscopy.

Abstract

A two-pinhole axially superresolving confocal fluorescence imaging system is presented. Based on the concept of subtractive incoherent imaging, the system described here is equipped with a zero-focus complex-transmittance pupil filter in one of the collector paths. The optical sectioning capacity of the system is 25% superior to that of a free-pupil one-pinhole instrument.

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BibTeXRIS

M Martínez-Corral, M T Caballero, C Ibáñez-López, V Sarafis. 2003. Optical sectioning by two-pinhole confocal fluorescence microscopy.. https://doi.org/10.1016/s0968-4328(03)00057-x

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