[Gene expression changes after heat shock by cDNA array analysis].

T Y Wang; N H Wu; Y F Shen

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PubMed · 12940077

[Gene expression changes after heat shock by cDNA array analysis].

T Y Wang·N H Wu·Y F Shen

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Abstract

OBJECTIVE: To study gene expression changes of cells in response to heat stress, we isolated total RNA from Jurkat cells before and after heat shock treatment. METHODS: cDNA was labeled with alpha-32P-dATP during reverse transcription of RNA and then used as probe to cDNA expression array. Autoradiogram images were analyzed by ESTblot software. RESULTS: After heat shock, the expression level of some forty genes increased while that of sixteen genes decreased. In addition to the elevated expression of heat shock genes, expression of c-Jun and CLK-1 increased most remarkably. The genes with notably decreased expression were integrin alpha-4 and transforming growth factor beta. The elevated expression of c-Jun and hsp90 alpha was further confirmed by Northern blot analysis. CONCLUSIONS: The expression of some genes in Jurkat cells changes after heat shock treatment. Heat shock induces elevated expression of hsp, c-Jun, CLK-1 gene while decreases expression of integrin alpha-4 and transforming growth factor beta.

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Blotting, Northern Caenorhabditis elegans Proteins Gene Expression Genes, jun Heat-Shock Proteins Heat-Shock Response Humans Integrin alpha Chains Jurkat Cells Oligonucleotide Array Sequence Analysis RNA, Messenger Transforming Growth Factor beta English Abstract Journal Article Research Support, Non-U.S. Gov't

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T Y Wang, N H Wu, Y F Shen. 2001. [Gene expression changes after heat shock by cDNA array analysis].. https://pubmed.ncbi.nlm.nih.gov/12940077/

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