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PubMed · 12951244

Characterization of species-specific genes using a flexible, web-based querying system.

Robert L Charlebois·G D Paul Clarke·Robert G Beiko·Andrew St Jean
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Abstract

We describe a query-based web-accessible system (www.neurogadgets.com/bws.php) for facilitating comparative microbial genomics. A variety of query pages are available, each with numerous options, that allow a biologist to pose relevant questions of genomic data. We illustrate with a characterization of species-specific protein-coding genes (so-called "ORFans"), finding that they are on average smaller, faster evolving, and less G+C-rich, and that they encode proteins more basic in their predicted isoelectric point, compared with non-species-specific genes. Using a dual-threshold approach, we conclude that these are characteristics of true species-specific genes, rather than artifacts of mis-annotation.

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Base CompositionComputational BiologyDatabases, GeneticGenetics, MicrobialGenomicsInternetOpen Reading FramesSpecies SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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Robert L Charlebois, G D Paul Clarke, Robert G Beiko, Andrew St Jean. 2003-08-29. Characterization of species-specific genes using a flexible, web-based querying system.. https://doi.org/10.1016/s0378-1097(03)00512-3

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Application of CE for determination of DNA base composition.

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