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PubMed · 12963967

Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens.

Wolfgang Hilbe·Margit Dlaska·Stephan Dirnhofer·Hans-Christoph Duba·Wolfgang Eisterer·Frieda Oberwasserlechner·Andreas Mildner·Thomas Schmid·Karlheinz Ammann·Josef Thaler·Ewald Wöll
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Abstract

The frequency of increased EGFR-mRNA expression was determined in 57 patients suffering from NSCLC by applying quantitative real-time PCR. The findings were correlated with clinical parameters and the immunohistochemical (IHC) markers EGFR, c-erbB-2, c-erbB-3, Ki-67 and p53 on cryostat sections. Of the patients 46% showed increased EGFR-mRNA, 35% revealed an increased IHC-EGFR expression; 16% of the patients showed a combined positivity and 35% a combined negativity when applying both methods, and 17 (30%) of the cases revealed increased EGFR-mRNA without IHC-EGFR expression. This subgroup was characterised by p53 coexpression and the highest frequency of deaths (35% vs. 20%) indicating a more aggressive tumour type. In contrast to IHC - where positivity was seen predominantly in squamous cell carcinomas (48% vs. 27%) - EGFR-mRNA expression was observed equally in both histological subtypes (48% vs. 43%). PCR-EGFR and IHC-EGFR tumour typing identifies different tumour characteristics with different clinical courses. Whether this combined typing could help to identify patients who respond to anti-EGFR therapies is worth further testing.

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Carcinoma, Non-Small-Cell LungErbB ReceptorsHumansImmunohistochemistryKi-67 AntigenLung NeoplasmsPolymerase Chain ReactionRNA, MessengerReverse Transcriptase Polymerase Chain ReactionTime FactorsTranscription, GeneticTumor Suppressor Protein p53Journal ArticleResearch Support, Non-U.S. Gov't

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Wolfgang Hilbe, Margit Dlaska, Stephan Dirnhofer, Hans-Christoph Duba, Wolfgang Eisterer, Frieda Oberwasserlechner, Andreas Mildner, Thomas Schmid, Karlheinz Ammann, Josef Thaler, Ewald Wöll. 2003. Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens.. https://pubmed.ncbi.nlm.nih.gov/12963967/

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