Temporal comparison of Broad-Complex expression during eggshell-appendage patterning and morphogenesis in two Drosophila species with different eggshell-appendage numbers.

Karen E James; Celeste A Berg

doi:10.1016/s1567-133x(03)00136-4

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Temporal comparison of Broad-Complex expression during eggshell-appendage patterning and morphogenesis in two Drosophila species with different eggshell-appendage numbers.

Karen E James·Celeste A Berg

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Abstract

A central question in biology is how developmental mechanisms are altered to bring about morphological evolution. Drosophilids boast a remarkable diversity in eggshell-appendage number-from as few as one to as many as nine, depending on the species. Appendage patterning in Drosophila melanogaster is well characterized, inviting candidate-gene-based approaches that identify the developmental mechanisms underlying Drosophilid eggshell diversity. Previous studies show that a combination of Epidermal growth factor receptor (EGFR) and TGFbeta/BMP2,4 Decapentaplegic (DPP) signaling determines appendage fate in D. melanogaster. Broad-Complex expression integrates EGFR and DPP signaling and predicts future appendage position. Here we present our confocal analyses of BR-C immunofluorescence and appendage morphogenesis in Drosophila melanogaster (two appendages) and Drosophila virilis (four appendages). Our comparison suggests that differences in BR-C patterns among Drosophilids may be strongly influenced by anterior-posterior information.

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Animals Body Composition Body Patterning Drosophila Drosophila Proteins Drosophila melanogaster Embryo, Nonmammalian Fluorescent Antibody Technique Gene Expression Profiling Microscopy, Confocal Morphogenesis Species Specificity Transcription Factors Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Karen E James, Celeste A Berg. 2003. Temporal comparison of Broad-Complex expression during eggshell-appendage patterning and morphogenesis in two Drosophila species with different eggshell-appendage numbers.. https://doi.org/10.1016/s1567-133x(03)00136-4

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