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Structural characterization and expression of a brain specific gene encoding chick kainate binding protein.

N Eshhar·C Hunter·R J Wenthold·K Wada
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Abstract

The gene encoding chick kainate-binding protein (c-KBP), a member of the non-NMDA ionotropic glutamate receptor family has been isolated and characterized. The c-KBP gene is at least 13 kilobases long and contains 11 exons interrupted by 10 introns. Primer extension and RNase protection studies identified a major transcription initiation site located 117 bases upstream from the initiation methionine codon ATG. Consensus TATA and CCAAT sequences were detected in the putative promoter region. The structure of the c-KBP gene is strikingly different from that of other members of neurotransmitter-gated ion-channels (cloned at present) although the topology of c-KBP consists of four membrane-spanning domains, a structural characteristic of ionotropic receptor subunits. The c-KBP gene was found to be expressed at high levels in chick cerebellar Bergmann glia and at extremely low levels in the forebrain. The limited expression of the c-KBP gene raises important questions concerning the mechanisms governing the regulation of c-KBP gene transcription.

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AnimalsBase SequenceBrainCerebellumChickensDNAExonsGene Expression RegulationIntronsKainic AcidMolecular Sequence DataNucleic Acid HybridizationOrgan SpecificityKainic Acid ReceptorsReceptors, NeurotransmitterTranscription, GeneticJournal Article

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N Eshhar, C Hunter, R J Wenthold, K Wada. 1992-02-10. Structural characterization and expression of a brain specific gene encoding chick kainate binding protein.. https://doi.org/10.1016/0014-5793(92)80551-q

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