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A continuous cell line (KK-2) from a supratentorial primitive neuroectodermal tumor.

H Ito·T Kameya·T Suwa·C Wada·N Kawano
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Abstract

Tumor tissue located in the occipital lobe with hemorrhage was obtained from a 19-year-old patient. Histological examination indicated it to consist of undifferentiated small, round cells without neuronal or glial differentiation, and possibly to be a type of primitive neuroectodermal tumor. The tumor cells were cultured for 3 years and a continuous cell line (KK-2) was established. KK-2 was transplantable to nude mice. With immunocytochemistry, neuron-specific enolase, protein gene product 9.5, vimentin, TUJ1 (a monoclonal antibody specific for neuron-associated class III beta-tubulin isotype) and 6H7 (a monoclonal antibody to NCAM produced by us) were detected. None of the following could be found: glial fibrillary acidic protein, S-100 protein, neurofilament and synaptophysin, calcitonin gene-related peptide, gastrin releasing peptide corticotropin-releasing factor, substance P, somatostatin, chromogranin, aromatic L-amino acid decarboxylase and tyrosine hydroxylase. The original tumor and KK-2 cells obtained after 3 years of culture and transplants in nude mice displayed essentially the same ultrastructural and immunohistochemical characteristics. KK-2 cells showed no differentiation to mature neuronal, glial or ependymal cells. This cell line may possibly serve as a useful model for studying cellular differentiation of human neuroectodermal tumors and normal neuronal development.

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AdultBrain NeoplasmsFemaleHumansImmunohistochemistryMicroscopy, ElectronNeoplasms, Germ Cell and EmbryonalOccipital LobeTumor Cells, CulturedCase ReportsJournal ArticleResearch Support, Non-U.S. Gov't

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H Ito, T Kameya, T Suwa, C Wada, N Kawano. 1992. A continuous cell line (KK-2) from a supratentorial primitive neuroectodermal tumor.. https://doi.org/10.1007/bf00427215

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