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Regulation of microglial function by interferons.

C A Colton·J Yao·J E Keri·D Gilbert
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Abstract

Cultured neonatal rat microglia were pretreated with varying doses of either purified interferon (IFN) alpha/beta or recombinant IFN gamma for 24 or 48 h and the following functional parameters examined; superoxide anion production, interleukin-1 secretion and chemotaxis. IFN gamma produced a marked increase in superoxide anion levels when PMA was used to initiate superoxide anion production but had no effect in OPZ-stimulated microglia. Treatment with IFN alpha/beta potentiated superoxide anion production in both PMA and OPZ-stimulated cells. Interleukin-1 activity was decreased by treatment with IFN gamma for 24 h while IFN alpha/beta increased IL-1 activity at 48 h. Removal of serum from the treatment media prevented the action of IFN alpha/beta on IL-1 production. Treatment with IFN alpha/beta or gamma decreased chemotaxis of microglia in response to zymosan activated serum. The data indicate that IFN gamma and alpha/beta can regulate microglial function and that this regulation may differ from that seen for other monocytically derived macrophages.

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AnimalsAnionsChemotaxisInterferon-alphaInterferon-betaInterferon-gammaInterleukin-1NeurogliaSuperoxidesJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.

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C A Colton, J Yao, J E Keri, D Gilbert. 1992. Regulation of microglial function by interferons.. https://doi.org/10.1016/0165-5728(92)90216-8

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