Improved method for coliphage detection based on beta-galactosidase induction.

M M Ijzerman; C Hagedorn

doi:10.1016/0166-0934(92)90004-w

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Improved method for coliphage detection based on beta-galactosidase induction.

M M Ijzerman·C Hagedorn

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Abstract

An improved method for coliphage detection based on the induction of beta-galactosidase in Escherichia coli is described. Upon infection by coliphages, the cells are lysed and a stable indolyl product that is dark blue becomes visible within each plaque. The improved method is compared to the proposed coliphage detection procedure described in Standard Methods for the Examination of Water and Wastewater.

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Coliphages Enzyme Induction Escherichia coli Galactosides Indoles Isopropyl Thiogalactoside Viral Plaque Assay Water Microbiology beta-Galactosidase Journal Article Research Support, Non-U.S. Gov't

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M M Ijzerman, C Hagedorn. 1992. Improved method for coliphage detection based on beta-galactosidase induction.. https://doi.org/10.1016/0166-0934(92)90004-w

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