PubMed · 13599701
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P SOUTEYRAND. 1958. [Chelating agents].. https://pubmed.ncbi.nlm.nih.gov/13599701/
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A two-thiol reactive lanthanide-DOTA (1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetic acid) chelate, CLaNP-3 (CLaNP=caged lanthanide NMR probe), was synthesized for the rigid attachment to cysteine groups on a protein surface, and used to obtain long-range-distance information from the {15N,1H} HSQC spectra of the protein-lanthanide complex. The DOTA ring exhibits several isomers that are in exchange; however, single resonances were observed for most amide groups in the protein, allowing determination of a single, apparent magnetic-susceptibility tensor. Pseudocontact shifts caused by Yb-containing CLaNP-3 were observed for atoms at 15-35 A from the metal. By using Gd-containing CLaNP-3, relaxation effects were observed, allowing distances up to 30 A from the paramagnetic center to be determined accurately. Similar results were obtained with a Gd-DTPA (diethylene-triaminepentaacetic acid) chelate, CLaNP-1, bound in the same bidentate manner to the protein. This study demonstrates that bidentate attachment of a paramagnetic probe enables determination of long-range distances.
rac-[Co(ambi)3]Cl3.3H2O corresponds to a tris-chelate complex bearing 2-(aminomethyl)benzimidazole (ambi) ligands bound to the Co(III) ion in a facial-type fashion. The high metal q/r ratio and electronic delocalization between coordination heterocyclic functionalities can provide powerful tools to minimize DNA condensation, which arises from the neutralization of the negatively charged sugar phosphate backbone in the presence of multivalent [Co(ambi)3]3+.
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