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A novel, quantitative bioassay for type I interferon using a recombinant indicator cell line.

R Lleonart·D Näf·H Browning·C Weissmann
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Abstract

We describe a specific and quantitative novel assay for biologically active human type I interferon (IFN), the MxR assay. It is based on a Vero cell line containing multiple copies of a hybrid gene consisting of the murine Mx promoter, which is responsive to type I IFN, linked to the human growth hormone (hGH) transcription unit. Exposure of this cell line to IFN-alpha or -beta for 12-48 hours results in the production of hGH that is measured by a commercially available radio-immune assay. The response to IFN-alpha is dose-dependent between 3 and 1000 units/ml. There is no response to TNF, IL-1 and a number of other cytokines and growth factors, and only a negligible response to IFN-gamma.

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AnimalsBiological AssayGTP-Binding ProteinsGrowth HormoneHumansInterferon Type IMyxovirus Resistance ProteinsPromoter Regions, GeneticProteinsRadioimmunoassaySensitivity and SpecificityTransfectionVero CellsJournal ArticleResearch Support, Non-U.S. Gov't

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R Lleonart, D Näf, H Browning, C Weissmann. 1990. A novel, quantitative bioassay for type I interferon using a recombinant indicator cell line.. https://doi.org/10.1038/nbt1290-1263

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