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A yeast biosensor for glucose determination.

J Racek
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Abstract

A yeast potentiometric biosensor for glucose determination is described. After induction of glycolytic enzyme synthesis a cell suspension of the yeast Hansenula anomala is retained in calcium alginate gel on the surface of a glass electrode. This biosensor gives a Nernstian response in glucose concentration of 5 x 10(-4)-5 x 10(-3) mol/l with a response time of 5 min and a life-time of at least 2 months. Mannose and fructose are the only significantly interfering substances. The biosensor was used for measurement of glucose concentration in urine with results comparable to those obtained by a photometric enzymatic method.

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Biological AssayFructoseGlucoseHydrogen-Ion ConcentrationMannoseTemperatureYeastsJournal Article

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J Racek. 1991. A yeast biosensor for glucose determination.. https://doi.org/10.1007/bf00180573

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