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New molecular biology methods for protein engineering.

M J Zoller
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Abstract

This review outlines recent advances in the application of molecular biological techniques to the study of protein structure and function. The chapter is divided into four main sections: methods for oligonucleotide-directed mutagenesis; mutational strategies for identifying functional residues and domains; systems for expression; and future developments. Few new methods were reported in 1990; however, a number of the papers that appeared represent refinements of previously reported strategies. This review is also published in Current Opinion in Structural Biology 1991, 1:605-610.

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M J Zoller. 1991. New molecular biology methods for protein engineering.. https://doi.org/10.1016/0958-1669(91)90076-h

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