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PubMed · 1367934

Semi-automated solid-phase DNA sequencing.

M Uhlén·T Hultman·J Wahlberg·J Lundeberg·S Bergh·B Pettersson·A Holmberg·S Ståhl·T Moks
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Abstract

Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template preparation, sequencing reactions, product separation and detection. A semi-automated system, whereby PCR-amplified biotinylated genomic or plasmid DNA is immobilized on streptavidin-coated magnetic beads, has been developed.

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AutomationBase SequenceCosts and Cost AnalysisDNAPlasmidsPolymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov'tReview

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M Uhlén, T Hultman, J Wahlberg, J Lundeberg, S Bergh, B Pettersson, A Holmberg, S Ståhl, T Moks. Semi-automated solid-phase DNA sequencing.. https://doi.org/10.1016/0167-7799(92)90169-v

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