Recombinant human insulin.

M R Ladisch; K L Kohlmann

doi:10.1021/bp00018a001

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PubMed · 1369033

Recombinant human insulin.

M R Ladisch·K L Kohlmann

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Abstract

Insulin is a well-characterized peptide that can be produced by recombinant DNA technology for human therapeutic use. A brief overview of insulin production from both traditional mammalian pancreatic extraction and recombinant bacterial and yeast systems is presented, and detection techniques, including electrophoresis, are reviewed. Analytical systems for insulin separation are principally based on reversed-phase chromatography, which resolves the deamidation product(s) (desamido insulin) of insulin, proinsulin, and insulin. Process-scale separation is a multistep process and includes ion exchange, reversed-phase, and size exclusion chromatography. Advantages and/or disadvantages of various separation approaches, as described by the numerous literature references on insulin purification, are presented.

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Amino Acid Sequence Animals Biotechnology Chromatography, High Pressure Liquid Humans Insulin Molecular Sequence Data Recombinant Proteins Sequence Homology, Amino Acid Species Specificity Swine Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Review

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M R Ladisch, K L Kohlmann. Recombinant human insulin.. https://doi.org/10.1021/bp00018a001

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