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PubMed · 1369647

[3H]zonisamide binding in rat brain.

T Mimaki·Y Suzuki·T Tagawa·T Karasawa·H Yabuuchi
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Abstract

We previously reported that zonisamide inhibits both [3H]flunitrazepam and [3H]muscimol binding in rat brain. In the present study, [3H]zonisamide was found to bind in a saturable fashion to the crude synaptosomal fraction of whole rat brain. Linear regression analysis of the binding data in the Scatchard plot indicated a Kd of 90 nM, and a maximal binding capacity of 1.40 x 10(3) fmol/mg protein. Displacement studies revealed an inhibitory effect of clonazepam and an enhancement effect of GABA on specific [3H]zonisamide binding. These results suggest that specific [3H]zonisamide binding sites may have a tight correlationship with benzodiazepine receptors in rat brain.

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AnimalsAnticonvulsantsBrainClonazepamDose-Response Relationship, DrugIsoxazolesMaleRatsRats, Sprague-DawleyReceptors, GABA-ASynaptosomesZonisamidegamma-Aminobutyric AcidJournal ArticleResearch Support, Non-U.S. Gov't

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T Mimaki, Y Suzuki, T Tagawa, T Karasawa, H Yabuuchi. 1990. [3H]zonisamide binding in rat brain.. https://pubmed.ncbi.nlm.nih.gov/1369647/

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