Exon skipping in human beta-casein.

R S Menon; Y F Chang; K F Jeffers; R G Ham

doi:10.1016/0888-7543(92)90400-m

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Exon skipping in human beta-casein.

R S Menon·Y F Chang·K F Jeffers·R G Ham

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Abstract

Earlier amino acid alignments of mature beta-caseins showed that the human protein was shifted in alignment relative to other species, with amino acid deletions in the N-terminal region and others inserted in the C-terminal region. Our alignment, based on cDNA sequences and their translation products, has shown that the amino acid deletions correspond exactly to exon 3 in the other species. Cloning and sequencing of a segment of the human beta-casein gene between exons 2 and 4 revealed the presence of an intact exon 3 sequence in the gene. An interruption of the polypyrimidine tract adjacent to the 5' end of exon 3 sequence may account for the omission of the exon from human beta-casein mRNA.

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Amino Acid Sequence Base Sequence Caseins Cloning, Molecular Exons Humans Molecular Sequence Data RNA Sequence Alignment Sequence Homology, Nucleic Acid Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R S Menon, Y F Chang, K F Jeffers, R G Ham. 1992. Exon skipping in human beta-casein.. https://doi.org/10.1016/0888-7543(92)90400-m

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