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Interleukin-7 expression during mouse thymus development.

M V Wiles·P Ruiz·B A Imhof
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Abstract

We have monitored the expression of interleukin-7 (IL-7) in the developing embryonic mouse thymus by a combination of quantitative polymerase chain reaction (PCR) and immunofluorescence microscopy. A strong specific signal for IL-7 mRNA was detected by day 12 in the developing fetal thymus. IL-7 mRNA was found to be maximally expressed on day 15, and then decreased over the next 5 days. Immunofluorescence staining of fetal thymus sections using an anti-IL-7 antibody confirmed these PCR data. IL-7 protein expression was first detected at day 13 of development. At 14 days the intensity of the staining increased by a factor of three and stayed at this level over the next 4 days. The same anti-IL-7 antibody used for immunofluorescence, blocked the proliferation of fetal thymocytes in organotypic cultures. In addition, we detected mRNA coding for IL-2 and SCF (also known as the steel factor or KL) in embryonic thymocytes. The implications of these findings for early thymocyte growth are discussed.

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AnimalsBase SequenceCadherinsFluorescent Antibody TechniqueGene ExpressionHematopoietic Cell Growth FactorsInterleukin-2Interleukin-7MiceMolecular Sequence DataOligodeoxyribonucleotidesPolymerase Chain ReactionRNA, MessengerStem Cell FactorThymus GlandJournal ArticleResearch Support, Non-U.S. Gov't

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M V Wiles, P Ruiz, B A Imhof. 1992. Interleukin-7 expression during mouse thymus development.. https://doi.org/10.1002/eji.1830220424

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