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PubMed · 1373487

Human combinatorial antibody libraries to hepatitis B surface antigen.

S L Zebedee·C F Barbas·Y L Hom·R H Caothien·R Graff·J DeGraw·J Pyati·R LaPolla·D R Burton·R A Lerner
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Abstract

Human antibody Fab fragments that bind to hepatitis B surface antigen (HBsAg) were generated by using a recombinant phage surface-display expression system. Characterization of HBsAg-specific Fab fragments isolated from two vaccinated individuals reveals diversity in specificity of antigen binding and in the sequences of the complementarity-determining region. The sequence results show examples of human light-chain promiscuity that result in fine specificity changes and a strong relationship to a human germ-line gene. This application illustrates further that this technique is a powerful tool to isolate distinct human antibodies against immunogenic viral targets.

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Amino Acid SequenceBase SequenceDNAEnzyme-Linked Immunosorbent AssayGene LibraryHepatitis B AntibodiesHepatitis B Surface AntigensHumansImmunoglobulin Fab FragmentsImmunoglobulin Heavy ChainsImmunoglobulin Light ChainsMolecular Sequence DataOligodeoxyribonucleotidesRNARecombinant ProteinsSequence Homology, Nucleic AcidComparative StudyJournal Article

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S L Zebedee, C F Barbas, Y L Hom, R H Caothien, R Graff, J DeGraw, J Pyati, R LaPolla, D R Burton, R A Lerner. 1992-04-15. Human combinatorial antibody libraries to hepatitis B surface antigen.. https://doi.org/10.1073/pnas.89.8.3175

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