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PubMed · 1373757

Immunological discrimination between the human apolipoprotein E2(Arg158----Cys) and E3 isoforms.

K Gerritse·P de Knijff·G van Ierssel·L M Havekes·R R Frants·M M Schellekens·N D Zegers·E Claassen·W J Boersma
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Abstract

A specific anti-apoE2(Arg158----Cys) monoclonal antibody was raised by means of immunization of mice with a variant specific synthetic peptide. The peptide sequences used were homologous to apolipoprotein E of human and mouse. Consequently, the mouse immune system was tolerant to most of the selected sequences. Immunization with only one of selected peptides (amino acids 154-172) evoked an anti-peptide and anti-native protein response. Surprisingly, this peptide was predicted to have a low antigenicity index, in contrast to the other used peptides. The variant specific anti-peptide MAb that was generated with this sequence, recognizes apoE2(Arg158----Cys) and not apoE3. We here describe a sensitive, time saving, and simple immunoblot assay to detect apoE2(Arg158----Cys) in human sera without prior isoelectric focusing of serum proteins.

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Amino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityApolipoprotein E2Apolipoprotein E3Apolipoproteins EArginineCysteineEnzyme-Linked Immunosorbent AssayEpitopesFemaleHumansImmunoblottingIsoelectric FocusingMiceMice, Inbred BALB CMolecular Sequence DataPhenotypeSequence AlignmentJournal Article

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K Gerritse, P de Knijff, G van Ierssel, L M Havekes, R R Frants, M M Schellekens, N D Zegers, E Claassen, W J Boersma. 1992. Immunological discrimination between the human apolipoprotein E2(Arg158----Cys) and E3 isoforms.. https://pubmed.ncbi.nlm.nih.gov/1373757/

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