Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.

C D Prescott; H C Kornau

doi:10.1093/nar/20.7.1567

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PubMed · 1374555

Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.

C D Prescott·H C Kornau

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Abstract

The in vivo expression of mutations constructed within helix 34 of 16S rRNA has been examined together with a nonsense tRNA suppressor for their action at stop codons. The data revealed two novel results: in contrast to previous findings, some of the rRNA mutations affected suppression at UAA and UAG nonsense codons. Secondly, both an increase and a decrease in the efficiency of the suppressor tRNA were induced by the mutations. This is the first report that rRNA mutations decreased the efficiency of a suppressor tRNA. The data are interpreted as there being competition between the two release factors (RF-1 and RF-2) for an overlapping domain and that helix 34 influences this interaction.

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Base Sequence Binding Sites Codon Escherichia coli Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Bacterial RNA, Ribosomal, 16S RNA, Transfer Journal Article

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C D Prescott, H C Kornau. 1992-04-11. Mutations in E.coli 16s rRNA that enhance and decrease the activity of a suppressor tRNA.. https://doi.org/10.1093/nar%2F20.7.1567

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