A two step stain for normal and leukemic monocytes using two different dyes applied in sequence.

L Kass

doi:10.3109/10520299209110010

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A two step stain for normal and leukemic monocytes using two different dyes applied in sequence.

L Kass

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Abstract

A staining procedure for monocytes in specimens of blood and bone marrow was developed. The technique was a two step procedure in which unfixed cells were exposed first to a methanolic solution of C.I. basic blue 54. Next, an aqueous alkaline buffered solution of C.I. basic blue 141 was added to the first staining solution. After staining for 10 min in the solution with two stains, slides or coverslips were washed for 5 sec in pH 5.6 phosphate buffer and drained dry. The cytoplasm of monocytes stained intensely deep purple and frequently nuclei were stained red. Similar staining was not found in other types of normal or abnormal blood and bone marrow cells.

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Buffers Cell Nucleus Chromatin Cytoplasm Erythroblasts Histocytological Preparation Techniques Humans Leukemia, Myeloid, Acute Monocytes Neutrophils Phosphates Precursor Cell Lymphoblastic Leukemia-Lymphoma Staining and Labeling Time Factors Journal Article

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L Kass. 1992. A two step stain for normal and leukemic monocytes using two different dyes applied in sequence.. https://doi.org/10.3109/10520299209110010

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