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A technique for embedding undecalcified bone samples for detecting alpha-emitters using vacuum impregnation with Spurr's resin.

J W Haines
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Abstract

A method has been developed by which large samples of mineralized bone, containing an alpha-emitter, can be embedded in Spurr's resin in a fraction of the time required by conventional methods. Bone samples were freeze-dried or fixed and dried prior to impregnation with Spurr's resin under vacuum. Sections were cut for the preparation of either alpha-track or fission-track autoradiographs using the solid state detector CR-39. This method is applicable to samples containing a mobile form of a radionuclide that may be translocated during the processes of fixation and dehydration of the specimen.

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Alpha ParticlesAnimalsBone and BonesGuinea PigsHumansMicePapioRadiumRatsResins, PlantStaining and LabelingSwineTissue EmbeddingVacuumComparative StudyJournal Article

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J W Haines. 1992. A technique for embedding undecalcified bone samples for detecting alpha-emitters using vacuum impregnation with Spurr's resin.. https://doi.org/10.3109/10520299209110005

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