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PubMed · 1382877

[A modified staining method for examining Y chromatin].

C Gao
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Abstract

A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differentiation diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 athletes for the 11th Asian Games.

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CheekFemaleHumansMaleMouth MucosaSex ChromatinSex Determination AnalysisStaining and LabelingY ChromosomeEnglish AbstractJournal Article

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C Gao. 1992. [A modified staining method for examining Y chromatin].. https://pubmed.ncbi.nlm.nih.gov/1382877/

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BACKGROUND: Impalement injuries of the soft palate and oropharynx are common in children, especially those of preschool age. These injuries are particularly common in toddlers, given their propensity to fall easily while carrying objects in their mouths. Although most of these injuries do not have lasting sequelae, some can have devastating neurological complications, and consequently, careful assessment of the patient during the early stages of trauma is imperative. Close follow-up for up to 72 h, and parental counselling and instruction should be considered as part of their immediate care. CASE REPORT: A case of toothbrush impalement in a 4-year-old child is presented. The foreign body was noted to be lodged in the cheek. After careful triaging, history taking and appropriate imaging, surgical removal of this brush was carried out uneventfully under general anaesthesia. No further complications were noted postoperatively, and because of the type of injury, the patient was discharged within 24 h and reviewed as an outpatient. CONCLUSION: A survey of the literature confirms that most injuries of this kind can be treated conservatively, and in many cases, without surgical intervention. However, careful assessment by an experienced clinician is necessary to rule out other complications. The present paper discusses the prevalence, management and complications associated with impalement injuries of the oral cavity in children.

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