Microplate coagulation assays.

C W Pratt; D M Monroe

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PubMed · 1389175

Microplate coagulation assays.

C W Pratt·D M Monroe

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Abstract

This report describes the development of microplate-based blood coagulation assays. The assays require a kinetic microplate reader to follow changes in absorbance at 405 nm caused by the coagulating plasma. Procedures for performing prothrombin time and activated partial thromboplastin time tests are described with intra- and inter-assay variability of a few percentage points. The prothrombin time of normal plasma was 64.5 +/- 3.6 s, and the activated partial thromboplastin time was 69.8 +/- 3.2 s. Clotting times were prolonged when normal plasma was mixed with plasmas deficient in particular coagulation factors, as expected. These assays take advantage of the microplate format (small sample size and multiple simultaneous assays) and can be customized for specific purposes, such as quantifying purified factor IX or assessing protein C activity in plasma.

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Blood Coagulation Tests Factor IXa Hemophilia B Humans Microchemistry Nephelometry and Turbidimetry Partial Thromboplastin Time Prothrombin Time Journal Article Research Support, U.S. Gov't, P.H.S.

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C W Pratt, D M Monroe. 1992. Microplate coagulation assays.. https://pubmed.ncbi.nlm.nih.gov/1389175/

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