PubMed · 13961166
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C H HIRS. 1962. Dinitrophenyiribonucleases.. https://pubmed.ncbi.nlm.nih.gov/13961166/
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In the presence of phosphomonoesterase contaminations the use of bis-p-nitrophenyl phosphate to measure phosphodiesterase activity gives inconclusive values because one of the products of the phosphodiesterase or nuclease reaction becomes a substrate of the contaminating enzyme. A direct determination of the hydrolyzed phosphodiesterase substrate in the UV range is possible at the isosbestic points of the transformation of the phosphomonoesterase substrate.
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