PubMed · 13999952
The source did not provide an abstract. Follow the original record for more information.
Explore connections, maps & timelines
H A WENNER. 1962-11-30. Problems in working with enteroviruses.. https://doi.org/10.1111/j.1749-6632.1962.tb18876.x
Cite the original work for its findings. Save a collection to share your selection of sources.
KEEP EXPLORING
Quantification of virus-like RNA sequences in biological fluids, like serum and cerebrospinal fluid, requires an RNA extraction method that is both reproducible and fast. Three RNA extraction methods were tested on enteroviruses: (1) the acid guanidine thiocyanate-phenol/chloroform (AGPC) method; (2) a method based on differential precipitation of the RNA and (3) a 'bind-wash-elute' system based on silica-gel membrane binding. The latter two methods yielded a comparable detection limit as measured by RT-PCR ELISA. The detection limit for the AGPC method was 10 times higher. The relative standard deviation for the bind-wash-elute method (3%) was superior to that of the other methods tested (both 20%) and provides a reliable and fast method to extract (viral) RNA from biological fluids for quantification by RT-PCR.
To determine the efficiency of reverse-transcriptase polymerase chain reaction (RT-PCR) assays currently used in diagnosing enterovirus and rhinovirus infections, we compared results obtained with RT-PCR methods, which detect both enteroviruses and rhinoviruses simultaneously, with results obtained by conventional virus isolation. Both tests were performed on 591 specimens: 38 samples (6%) had positive results by both RT-PCR and isolation, 90 samples (15%) had positive results by RT-PCR only, and 7 samples (1%) had positive results only by virus isolation. In conclusion, RT-PCR was superior in rapidity and sensitivity to virus isolation and is recommended as the primary diagnostic tool for enterovirus and rhinovirus infections.
During summer and fall, enterovirus infections are responsible for a considerable proportion of hospitalizations of young infants. We prospectively studied the incidence of enterovirus infections via real-time polymerase chain reaction (PCR) in blood, feces, and cerebrospinal fluid samples from infants <or=60 days old who had received a clinical diagnosis of sepsis. Forty-five patients were included: 19 were admitted to the pediatric wards of 2 general hospitals, and 26 had been hospitalized since birth in the neonatal intensive care unit (NICU) of a tertiary care hospital. None of the NICU patients developed enteroviral disease. In contrast, an enterovirus was detected in 11 (58%) of the patients admitted to the 2 general hospitals, 10 of whom (53%) showed evidence of systemic infection. Enterovirus infections are an important cause of sepsis in infants admitted to the hospital. Real-time PCR in serum was a rapid and sensitive method for diagnosis of enterovirus infection.