[Mycoplasma restriction-modification system MunI and its possible role in pathogenesis processes].

P S Stakenas; N M Zaretskaia; Z P Manelene; M M Mauritsas; V V Butkus; A A Ianulaĭtis

PubMed · 1406610

[Mycoplasma restriction-modification system MunI and its possible role in pathogenesis processes].

P S Stakenas·N M Zaretskaia·Z P Manelene·M M Mauritsas·V V Butkus·A A Ianulaĭtis

Abstract

The restriction-modification system, named RMMunI, has been purified and characterised from Friend murine erythroleukemia cells. The site-specific endonuclease recognizes and cleaves the 5'C1AATTG nucleotide sequence. RMunI is an isoschizomer of RMfeI from Mycoplasma fermentans. Site-specific methylase modifies the second adenine residue in the same sequence (5'Cam6ATTG). It was established that the discovered enzymatic system is from mycoplasma which contaminates cell lines. Mycoplasma's DNA hybridizes with species-specific DNA probed for Mycoplasma fermentans and Mycoplasma arginini. The possible role of mycoplasmic restriction-modification enzymes in the process of acquired immune deficiency syndrome are discussed.

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Acquired Immunodeficiency Syndrome Animals Bacteriophage lambda Base Sequence Chromatography, High Pressure Liquid Chromatography, Thin Layer DNA, Bacterial DNA, Viral Deoxyribonucleases, Type II Site-Specific Electrophoresis, Agar Gel Leukemia, Erythroblastic, Acute Mice Molecular Sequence Data Mycoplasma fermentans Tumor Cells, Cultured English Abstract Journal Article

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P S Stakenas, N M Zaretskaia, Z P Manelene, M M Mauritsas, V V Butkus, A A Ianulaĭtis. [Mycoplasma restriction-modification system MunI and its possible role in pathogenesis processes].. https://pubmed.ncbi.nlm.nih.gov/1406610/

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