Three-dimensional cell cultures mimic tissues.

W M Saltzman; M R Parkhurst; P Parsons-Wingerter; W H Zhu

doi:10.1111/j.1749-6632.1992.tb42590.x

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Three-dimensional cell cultures mimic tissues.

W M Saltzman·M R Parkhurst·P Parsons-Wingerter·W H Zhu

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Abstract

Three-dimensional cell culture using gels of type I collagen is a flexible method for studying cell behavior in a tissuelike environment. With only small changes in the basic protocol, we were able to encapsulate neutrophils, hepatocytes, and PC12 cells. As demonstrated by cell-specific assays for migration, protein secretion, and growth factor induction, the encapsulated cells were viable and functional. In future studies, we will focus on using these cell cultures to study cell movement, cell growth, and cell function in carefully controlled tissuelike environments.

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Animals Cell Adhesion Cell Division Cells, Cultured Chickens Culture Media Culture Techniques Humans Kinetics Liver Nerve Growth Factors Neutrophils PC12 Cells Serum Albumin Time Factors Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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W M Saltzman, M R Parkhurst, P Parsons-Wingerter, W H Zhu. 1992-10-13. Three-dimensional cell cultures mimic tissues.. https://doi.org/10.1111/j.1749-6632.1992.tb42590.x

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