Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.

E T Marques; J L Lima Filho

doi:10.1007/bf02922149

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PubMed · 1416950

Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.

E T Marques·J L Lima Filho

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Abstract

A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protection. A low-cost, home-made oxygen electrode was applied as a transducer. The system has sensitivity from 62.5 up to 500 microM of ascorbic acid with satisfactory operation for more than 2 mo.

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Alkylation Amines Ascorbate Oxidase Ascorbic Acid Biosensing Techniques Enzymes, Immobilized Microspheres Journal Article Research Support, Non-U.S. Gov't

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E T Marques, J L Lima Filho. Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.. https://doi.org/10.1007/bf02922149

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