PubMed · 14183637
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R R BRITO, A A FERREIRA, L L DE MORAES. [HYPERTONIC SOLUTIONS IN UROLOGY].. https://pubmed.ncbi.nlm.nih.gov/14183637/
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Displacement of one electrolyte solution by another in a microchannel is required in many biolab chip devices. The objective of this paper is to develop a better understanding of the displacement process between two electrolyte solutions under an applied electric field in a cylindrical microchannel in terms of the traveling distance of the interface between these two electrolyte solutions. In order to develop a general model to predict the location of the interface, two different situations are considered; one model assumes the presence of a sharp interface between the two solutions and the other model considers a mixing zone between the two solutions. Carefully conducted experiments were carried out to obtain the current-time relationship, which is used in the model to predict the location of the interface. In these experiments, deionized ultrafiltered water (DIUF water), 10 mM KCl, 0.1 mM KCl, and 0.1 mM LaCl3 solutions were used as the testing liquids. Polyamide-coated silica capillary tubes of internal diameter 100 mum and length 10 cm were employed in this study. The relationship between traveled distance of the interface and time was predicted by a developed model based on the measured current-time relationship for such a displacement process under a constant applied electric field. The characteristics of the nonlinear change of the traveling distance with the time were also discussed in this paper.
The kinetics of osmotic water permeability in proteoliposomes containing alpha 1-acid glycoprotein was investigated by means of stopped-flow spectrophotometry. A biphasic time-course of scattered light with time was registered. The rate constants calculated from fits to an exponential function in the first phase were proportional to the final medium osmolarity. The apparent second order rate constants Kapp (Osm-1 sec-1) were determined at different glycoprotein concentrations in the original mixture for preparation of proteoliposomes. The value of Kapp at lipid:glycoprotein weight ratio = 1 was plotted in Arrhenius coordinates. The calculated activation energy for water permeation through the lipid bilayer suggests that eventual channel mechanism may be involved due to the presence of glycoprotein molecule in the liposomes.
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