PubMed · 14185385
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E PETTE. 1964. [DEMYELINATION AS A MEMBRANE PROBLEM].. https://pubmed.ncbi.nlm.nih.gov/14185385/
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Protein analysis techniques are developing fast due to the growing number of proteins obtained by recombinant DNA techniques. In the present paper we compare selected techniques, which are used for protein sizing, quantitation and molecular weight determination: sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), lab-on-a-chip or microfluidics technology (LoaC), size exclusion chromatography (SEC) and mass spectrometry (MS). We compare advantages and limitations of each technique in respect to different application areas, analysis time, protein sizing and quantitation performance.
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The chromatographic behavior of three naphthalimide-type stationary phases were elucidated in terms of hydrophobic, silanophilic and pi-pi interaction properties, employing besides common chromatographic column test methods from Engelhardt and Tanaka, also new test mixtures of geometrical and functional aromatic isomers. It was found that the presence of electron donor/acceptor moieties within a reversed phase system did not only increase the overall retention times for aromatic solutes, but also lead to an enhanced shape selectivity of the hybrid stationary phase. In this context, shape discrimination is primarily based on the number of accessible pi-electrons for pi-pi interaction with the embedded electron deficient ligand moieties. The most outstanding results were obtained for the 1,4,5,8-naphthalenediimidic selector with its horizontal arrangement on the silica surface, which enables a direct face-to-face pi-pi interaction with aromatic solutes, with only little hydrophobic contribution.