A simple and rapid method for screening transformant yeast colonies using PCR.

Q Liang; T Richardson

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PubMed · 1418974

A simple and rapid method for screening transformant yeast colonies using PCR.

Q Liang·T Richardson

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Abstract

A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification.

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Base Sequence Cloning, Molecular DNA, Recombinant Electrophoresis, Agar Gel Humans Lactoferrin Molecular Sequence Data Plasmids Polymerase Chain Reaction Saccharomyces cerevisiae Transformation, Genetic Journal Article Research Support, Non-U.S. Gov't

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Q Liang, T Richardson. 1992. A simple and rapid method for screening transformant yeast colonies using PCR.. https://pubmed.ncbi.nlm.nih.gov/1418974/

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