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PubMed · 1419882

Optimized amplification of the polymorphic system COL2A1.

K Skowasch·B Brinkmann
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Abstract

The influence of various amplification parameters on the demonstration of COL2A1 patterns was examined in serial experiments. The combination of 6 optimized parameters (concentration of primers, nucleotides, Taq polymerase, K+, Mg2+, number of cycles) led to an approximately tenfold increase in sensitivity and a decrease in allelic drop-out. In unequal mixtures of DNA from 2 individuals the weakest component was detectable in dilutions down to 1:20. In a small population sample (n = 120) 10 alleles could be demonstrated.

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Electrophoresis, Polyacrylamide GelEvaluation Studies as TopicForensic MedicineGene AmplificationHumansPolymerase Chain ReactionPolymorphism, GeneticRepetitive Sequences, Nucleic AcidJournal Article

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K Skowasch, B Brinkmann. 1992. Optimized amplification of the polymorphic system COL2A1.. https://doi.org/10.1007/bf01625175

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