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A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.

B T Farmer·R A Venters·L D Spicer·M G Wittekind·L Müller
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Abstract

A 3D optimized, refocused HNCA experiment is described. It is demonstrated to yield a dramatic increase in sensitivity when applied to [13C, 15N]-labeled human carbonic anhydrase II, a 29-kDa protein. The reasons for the gain in sensitivity are discussed, and 3 distinct areas for further development are indicated.

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Carbon IsotopesCarbonic AnhydrasesHumansIsoenzymesMagnetic Resonance SpectroscopyMathematicsNitrogen IsotopesProtein ConformationJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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B T Farmer, R A Venters, L D Spicer, M G Wittekind, L Müller. 1992. A refocused and optimized HNCA: increased sensitivity and resolution in large macromolecules.. https://doi.org/10.1007/bf01875530

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