PubMed · 14227661
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F C ROSE. 1964. OPTIC ATROPHY.. https://doi.org/10.1136/pgmj.40.470.692
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In the last decade, various polymerase chain reaction (PCR)-based methods have been developed using ribosomal RNA (rRNA) for the identification of medically important fungi. In the present study, large subunit (LSU) and small subunit (SSU) of fungal rRNA were amplified and analyzed by single-stranded conformation polymorphism (SSCP) of nested PCR, restriction digestion, and SSCP of digested products. The relationship between several clinical isolates of patients suffering from aspergillosis, candidiasis, cryptococcosis, keratitis, and skin and nail infections has been established with standard fungal cultures using the SSU- and LSU-specific primers. Single-stranded conformation polymorphism of restriction profile of amplified products of LSU-specific primers was successfully used to differentiate fungi up to genus and species level.
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Amplified Fragment Length Polymorphism (AFLP) analysis was used for characterising 13 native Colombian Clostridium spp strains. The DNA extraction method was optimised and the use of cetyl trimethyl ammonium bromide (CTAB) and sodium chloride (NaCl) was incorporated. All strains could be typed in these conditions. The AFLP profiles obtained were submitted to multivariate analysis and compared with previous pulsed field gel electrophoresis (PFGE) results. The results suggested that the set of native strains could correspond to two new species different to those having been described to date. It is proposed that DNA-DNA hybridisation analysis should be done to produce complementary information for describing the new species.