INTERRELATIONSHIP BETWEEN STRUCTURE AND FUNCTION IN HEMOGLOBIN AND MYOGLOBIN.

In this study, we investigated the susceptibility to enzymatic and alkaline hydrolysis of diloxanide furoate (DF) and its cyclodextrin inclusion complexes, in aqueous solution. The cyclodextrins (CDs) utilized were beta-cyclodextrin (beta-CD), (2,6-di-O-methyl)-beta-cyclodextrin (DM-beta-CD) and (2,3,6-tri-O-methyl)-beta-cyclodextrin (TM-beta-CD). All cyclodextrins studied provided a stabilizing effect to diloxanide furoate hydrolysis. In alkaline hydrolysis (pH 10.75), without the enzyme, beta-CD and TM-beta-CD provided similar effect on the stability of DF, with an inhibition factor in the order of 2.0. The DM-beta-CD, on the other hand, provided more pronounced stabilization effect than the other two CDs, with an inhibition factor around of 8. The maximum activity of the enzyme occured around pH 7.0. In the presence of enzyme, all cyclodextrins produced similar effect, with a DF hydrolysis inhibition factor in the order of 10. However, the plot of rate of hydrolysis versus [CD] fit with a equation based in a model that considers the association of the enzyme with the CDs. Therefore, it is concluded that the stabilization of DF is not only due to its cyclodextrin complex but also due to enzyme inhibition by cyclodextrin complexation.

Chemical Phenomena↗

PubMed2003

Exploring selectivity requirements for COX-2 versus COX-1 binding of 3,4-diaryloxazolones using E-state index.

Considering the importance of developing selective COX-2 inhibitors, the present paper explores selectivity requirements for COX-2 versus COX-1 binding of 3,4-diaryloxazolones using electrotopological state (E-state) index. The study also shows the utility of E-state index in developing statistically acceptable model having direct physicochemical significance: electron density distribution of different atoms of the oxazolone ring and attached two phenyl rings are important for the selective binding with COX-2 over COX-1. Moreover, the use of indicator variable shows that presence of ortho R(1) substituent (except fluoro) on the N(3)-phenyl ring decreases COX-2 selectivity. Further, an amino substituent at R(2) position (i.e., sulfonamide compound) is favorable for increasing COX-2 selectivity when the R(3) position is unsubstituted.

Chemical Phenomena↗

PubMed2003

Analytical characterization of Moringa oleifera seed oil grown in temperate regions of Pakistan.

The hexane-extracted oil content of Moringa oleifera seeds ranged from 38.00 to 42.00%. Protein, fiber, and ash contents were found to be 26.50-32.00, 5.80-9.29, and, 5.60-7.50%, respectively. Results of physical and chemical parameters of the extracted oil were as follows: iodine value, 68.00-71.80; refractive index (40 degrees C), 1.4590-1.4625; density (24 degrees C), 0.9036-0.9080 mg/mL; saponification value, 180.60-190.50; unsaponifiable matter, 0.70-1.10%; and color (1 in. cell), 0.95-1.10 R + 20.00-35.30 Y. Tocopherols (alpha, gamma, and delta) in the oil were up to 123.50-161.30, 84.07-104.00, and 41.00-56.00 mg/kg, respectively. The oil was found to contain high levels of oleic acid (up to 78.59%) followed by palmitic, stearic, behenic, and arachidic acid up to levels of 7.00, 7.50, 5.99, and 4.21%, respectively. The induction period (Rancimat, 20 L/h, 120 degrees C) of the crude oil was 9.99 h and reduced to 8.63 h after degumming. Specific extinctions at 232 and 270 nm were 1.70 and 0.31, respectively. Many parameters of M. oleifera oil indigenous to Pakistan were comparable to those of typical Moringa seed oils reported in the literature. The results of the present analytical study were also compared with those of different vegetable oils.

Chemical Phenomena↗