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PubMed · 1425951

O-acylated heparin derivatives with low anticoagulant activity decrease proliferation and increase alpha-smooth muscle actin expression in cultured arterial smooth muscle cells.

T Bârzu·A Desmoulière·J M Herbert·M Level·J P Herault·M Petitou·J C Lormeau·G Gabbiani·M Pascal
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Abstract

Selectively O-acylated derivatives of various glycosaminoglycans were prepared and tested in vitro for their anticoagulant activity and their antiproliferative effect on rat and rabbit smooth muscle cells. When O-acylation (butyrylation or hexanoylation) had been performed on periodate-depolymerized heparin fragments having very low anticoagulant activity, the antiproliferative potency was markedly increased (IC50 = 2 and 1 micrograms/ml respectively, versus 31 micrograms/ml for starting compound) without an increase in anticoagulant activity. The antiproliferative activity was related to the degree of acylation. The O-acylated derivatives of heparin fragments were also very active in reversing the de-differentiation of smooth muscle cell in culture, as estimated by the increase in the expression of alpha-smooth muscle actin and alpha-smooth muscle actin mRNA.

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ActinsAcylationAnimalsAorta, ThoracicBlotting, NorthernBlotting, WesternCell DivisionCells, CulturedElectrophoresis, Polyacrylamide GelFluorescent Antibody TechniqueGlycosaminoglycansHeparinIn Vitro TechniquesMuscle, Smooth, VascularRNA, MessengerRabbitsRatsRats, Sprague-DawleyJournal ArticleResearch Support, Non-U.S. Gov't

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T Bârzu, A Desmoulière, J M Herbert, M Level, J P Herault, M Petitou, J C Lormeau, G Gabbiani, M Pascal. 1992-08-25. O-acylated heparin derivatives with low anticoagulant activity decrease proliferation and increase alpha-smooth muscle actin expression in cultured arterial smooth muscle cells.. https://doi.org/10.1016/0014-2999(92)90300-s

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