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PubMed · 1426385

Freezing cocultured human blastocysts.

Y Ménézo·B Nicollet·N Herbaut·D André
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Abstract

OBJECTIVE: To determine the possibility of obtaining good pregnancy rates (PRs) after freezing and thawing cocultured blastocysts. DESIGN: Human blastocysts were frozen first according to a protocol available from literature. Two other protocols including the addition of glycerol were designed to improve the results. SETTING: All the patients were under clinical management at the Institut Rhonalpin pour l'Etude de la Reproduction Humaine in Lyons, France. PATIENTS: Patients involved in the in vitro fertilization program have had their supernumerary embryos frozen according to the three protocols presented here. MAIN OUTCOME MEASURES: Embryo recovery after freezing and thawing. Clinical and ongoing pregnancies after embryo transfer (ET). RESULTS: A protocol including sucrose addition and reduction of steps in the preparation of the blastocysts for freezing gave us a 21% PR per transfer (15 ongoing) of 101 transfers (106 thawings). CONCLUSIONS: Freezing of cocultured human blastocysts allow good PRs. This can represent an alternative for repeated failures of ETs at early stages.

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BlastocystCryopreservationCulture TechniquesEmbryo ImplantationEmbryo TransferFemaleFertilization in VitroFreezingHumansPregnancyJournal Article

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BibTeXRIS

Y Ménézo, B Nicollet, N Herbaut, D André. 1992. Freezing cocultured human blastocysts.. https://doi.org/10.1016/s0015-0282(16)55445-4

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PubMed2006

[Sequential assessment of individually cultured embryos in IVF].

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